A unique property of mammalian oocyte is that its development after prophase I occurs in the absence of transcription and relies solely on regulation of mRNA stability and translation in space and time, which makes oogenesis very attractive from the view of RNA biology. Translation activation does not rely exclusively on a cytoplasmic polyadenylation process. Instead, a range of various translation initiation factors and translation repression proteins play an important role. Polysome profiling is an ultimate method for distinguishing stored maternal mRNA pool from mRNAs being translationally activated. We have recently developed polysome fractionation method, which allowed us to analyze polysome-bound mRNAs by RNAseq from scarce samples. This project is focused on the study of the differences in temporal global pattern of translation of specific transcripts in the MII oocytes maturated in vitro versus in vivo, as well as in zygotes obtained after in vivo fertilization versus zygotes after in vitro fertilization. The project aims to decipher roles of non-canonical translation initiation factors in regulation of translation of selected mRNAs during oocyte maturation and early embryogenesis using polysome profiling and RNAseq analysis as pivotal methods. The research is carried out in cooperation with Institute of Animal Physiology and Genetics CAS.
Koncicka, M., A. Tetkova, D. Jansova, E. Del Llano, L. Gahurova, J. Kracmarova, S. Prokesova, T. Masek, M. Pospisek, A. W. Bruce, M. Kubelka and A. Susor (2018). "Increased Expression of Maturation Promoting Factor Components Speeds Up Meiosis in Oocytes from Aged Females." Int J Mol Sci 19(9).
Mrvova, S., K. Frydryskova, M. Pospisek, V. Vopalensky and T. Masek (2018). "Major splice variants and multiple polyadenylation site utilization in mRNAs encoding human translation initiation factors eIF4E1 and eIF4E3 regulate the translational regulators?" Mol Genet Genomics 293(1): 167-186.
Frydryskova, K., T. Masek, K. Borcin, S. Mrvova, V. Venturi and M. Pospisek (2016). "Distinct recruitment of human eIF4E isoforms to processing bodies and stress granules." BMC Mol Biol 17(1): 21.
Mašek, T., L. Valášek and M. Pospíšek (2011). "Polysome analysis and RNA purification from sucrose gradients." Methods in molecular biology (Clifton, N.J.) 703: 293-309.
Mokrejs, M., T. Masek, V. Vopalensky, P. Hlubucek, P. Delbos and M. Pospisek (2010). "IRESite-a tool for the examination of viral and cellular internal ribosome entry sites." Nucleic Acids Research 38: D131-D136.
GA ČR 19-13491S (2019-2021) Kultivace oocytů in vitro vs. vývoj oocytů in vivo – je jejich fyziologie opravdu srovnatelná?
GA ČR 19-13491S
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